学术报告 11

Inhibition of N-nitrosobenzylmethylamine metabolism and DNA binding in cultured rat esophagus by ellagic acid.

鞣花酸抑制大鼠食道中 N-亚硝基苄基甲胺代谢和 DNA 结合的研究。

原文链接官方网址：https://www.ncbi.nlm.nih.gov/pubmed

Carcinogenesis. 1988 Jul;9(7):1313-6.

致癌作用。1988 年 7 月; 9（7）：1313-6。

Mandal S1, Shivapurkar NM, Galati AJ, Stoner GD.

Author information

Department of Pathology, Medical College of Ohio, Toledo 43699.

 作者信息

俄亥俄州医学院病理系，托莱多 43699

Abstract

The effect of ellagic acid (EA), a naturally occurring plant phenol, on the metabolism, DNA binding and DNA adduct formation of

N-nitrosobenzylmethylamine (NBMA) in cultured explants of rat esophagus was investigated. Explants were incubated in medium containing EA at non-toxic concentrations of 10, 50 and 100 microM for 16 h, followed by the addition of 1

microM [3H]NBMA and EA for 12 h. Explant DNA was isolated by phenol extraction

and hydroxylapatite chromatography, and benzaldehyde formation was determined by HPLC analysis of the culture medium. EA produced a significant inhibition in the total covalent binding of NBMA metabolites to DNA and in the production of benzaldehyde in the medium. After acid hydrolysis of the isolated DNA, the NBMA--DNA adducts were separated by HPLC. EA caused a

dose-dependent decrease in the formation of N7-methylguanine and

O6-methylguanine adducts. These results suggest that EA inhibits both the metabolism of NBMA and the binding of NBMA metabolites to DNA in cultured rat esophagus.

研究了鞣花酸（EA）（一种天然存在的植物酚）对大鼠食管培养外植体中       N-亚硝基苄基甲基胺

（NBMA）的代谢，DNA 结合和 DNA 加合物形成的影响。将外植体在含有 EA 的无毒浓度为 10,50 和 100μM 的培养基中温育 16 小时，然后加入 1μM[3 H] NBMA 和 EA 12 小时。通过苯 酚提取和羟基磷灰石色谱分离外植体 DNA，并通过培养基的 HPLC 分析测定苯甲醛的形成。EA 对 NBMA 代谢产物与 DNA 的总共价结合以及培养基中苯甲醛的产生产生了显着的抑制作用。 在分离的 DNA 酸水解后，通过 HPLC 分离 NBMA-DNA 加合物。EA 引起 N7-甲基鸟嘌呤和 O6-甲基鸟嘌呤加合物形成的剂量依赖性降低。这些结果表明 EA 抑制 NBMA 的代谢和 NBMA 代谢物与培养的大鼠食道中的 DNA 的结合。

PMID:3383347

源自：

美国国家医学图书馆

美国国家卫生研究院

US National Library of Medicine/

Us National Institutes of Health/