学术报告 4

Cyanidin-3-glucoside extracted from mulberry fruit can reduce N-methyl-N-nitrosourea-induced retinal degeneration in rats

桑果中提取的花青素矢车菊色素3-葡萄糖苷可减轻N-甲基-N-亚硝基 脲诱导的大鼠视网膜变性实验

原文链接官方网址：https://www.ncbi.nlm.nih.gov/pubmed

Curr Eye Res. 2014 Jan;39(1):79-87. doi: 10.3109/02713683.2013.825275. Epub 2013 Jul 24.

 Abstract  摘要

Purpose: To investigate the effect of cyanidin-3-O-glucoside (C3G) on a rat retinal degeneration (RD) model.

目的：探讨花青素矢车菊色素3-葡萄糖苷(简称：C3G)对大鼠视网膜变性(简称：RD)模型的 影响。

Materials and methods: Experimental RD was induced in rats by the intraperitoneal injection of N-methyl-N-nitrosourea (MNU) at 50 mg/kg. C3G extracted from mulberry (Morus alba L.) fruit (50 mg/kg) was orally administered, daily for 1, 2 and 4 weeks after MNU injection. The effects of C3G administration on MNU-induced RD retinas were histologically and functionally assessed by hematoxylin and eosin staining and electroretinography (ERG), respectively. The degree of retinal injury in C3G-administered RD rats was evaluated by immunohistochemistry with an antibody against glial fibrillary acidic protein (GFAP). The preferential protective effect of C3G on scotopic vision was examined by western blot analysis.

材料和方法：大鼠腹腔注射N-甲基-N-亚硝基脲(MNU)50 mg/kg诱导实验性RD。从桑树 (Morus alba L.)果品中提取的50 mg/kg，每日通过注射MNU后，持续1、2、4周并进行观 察。分别用苏木精、伊红染色和视网膜电图(ERG)观察C3G对MNU诱导RD视网膜的组织学 和功能的影响。用抗胶质纤维酸性蛋白(GFAP)抗体免疫组织化学方法观察C3G大鼠视网膜 损伤程度。Westernblot分析C3G对暗视的优先保护作用。

Results: Marked loss of photoreceptors in the outer nuclear layer (ONL) was observed in RD rats at 2 and 4 weeks after MNU injection, while the ONL in the MNU-induced RD rats given C3G was relatively well preserved.

Immunohistochemistry with anti-GFAP showed that retinal injury was also reduced in the retinas of the rats given C3G. Functional assessment by using ERG recordings showed that scotopic ERG responses were significantly increased in RD rats given C3G for 4 weeks (p < 0.01) compared with that of untreated RD rats. In the RD rats given short-term C3G (for 1 and 2 weeks), the increase in ERG responses was not significant. In addition, western blot analysis showed that rhodopsin level in the C3G-administered RD retinas significantly increased compared to that in the non- administered RD retinas (p < 0.05), whereas red/green opsin level did not show any significant difference.

 结果：注射MNU后2、4周RD大鼠外核层(ONL)光感受器明显丢失，而MNU诱导的RD大鼠 ONL保存较好。抗GFAP免疫组化显示C3G大鼠视网膜损伤也明显减轻。用ERG记录功能评 估显示，与未治疗的RD大鼠相比，C3G大鼠4周后暗视ERG反应显著增加(P<0.01)。在短期 给予C3G(1周和2周)的RD大鼠，ERG反应无明显增加。此外，westernblot分析显示，C3G 处理的RD视网膜视紫红质水平明显高于未给药RD视网膜(p<0.05)，而红绿视黄蛋白水平无显著性差异（P>0.05）

Conclusions: Long-term administration of C3G extracted from mulberry fruit could structurally reduce photoreceptor damage and functionally improve scotopic visual functions in the RD rat model induced by MNU.

 结论：从桑果中提取的C3G（花青素矢车菊色素3-葡萄糖苷）能在MNU诱导的RD大鼠模型 中结构上减轻光感受器损伤，改善暗视功能。

Authors

Seung Hee Lee 1 , Eojin Jeong, Sun-Sook Paik, Ji Hyun Jeon, Sung Won Jung, Hyun-Bok Kim, Muyan Kim, Myung-Hoon Chun, In-Beom Kim Affiliation

1 Department of Anatomy . PMID: 23883132

DOI: 10.3109/02713683.2013.825275

源自：

美国国家医学图书馆

美国国家卫生研究院

US National Library of Medicine/

Us National Institutes of Health/