Ulcerative colitis (UC) is a chronic inflammatory disease of the colonic mucosa that can dramatically increase the risk of colon cancers. In the present study, we evaluated the effects of a dietary intervention of freeze-dried black raspberries

(BRB), a natural food product with antioxidant and anti-inflammatory bioactivities,

on disease severity in an experimental mouse model of UC using 3% dextran sodium sulfate (DSS). C57BL/6J mice were fed either a control diet or a diet containing BRB (5 or 10%) for 7-14 days and then the extent of colonic injury was assessed. Dietary BRB markedly reduced DSS-induced acute injury to the colonic epithelium. This protection included better maintenance of body mass and reductions in colonic shortening and ulceration. BRB treatment, however, did not affect the levels of either plasma nitric oxide or colon malondialdehyde, biomarkers of oxidative stress that are otherwise increased by DSS-induced colonic injury. BRB treatment for up to 7 days suppressed tissue levels of several key pro-inflammatory cytokines, including tumor necrosis factor α and interleukin 1β. Further examination of the inflammatory response by western blot analysis revealed that 7 day BRB treatment reduced the levels of phospho-IκBα within the colonic tissue.

Colonic cyclooxygenase 2 levels were also dramatically suppressed by BRB treatment, with a concomitant decrease in the

plasma prostaglandin E₂ (276 versus 34 ng/ml). These findings demonstrate a potent anti-inflammatory effect of BRB

during DSS-induced colonic injury, supporting its possible therapeutic or preventive role in the pathogenesis of UC and related neoplastic events.

溃疡性结肠炎（UC）是结肠粘膜的慢性炎症性疾病，其可以显着增加结肠癌的风险。在本研究中，我们评估了冷冻干燥黑覆盆子（BRB）（一种具有抗氧化和抗炎生物活性的天然食物产品）在 UC 的实验小鼠模型中使用 3％葡聚糖钠硫酸盐（DSS）。给予 C57BL / 6J 小鼠对照饮食或治疗，不影响血浆一氧化氮或结肠丙二醛的水平，氧化应激的生物标志物由 DSS 诱导的结肠损伤增加。BRB 治疗达 7 天可抑制几种关键的促炎细胞因子，包括肿瘤坏死因子 α 和白细胞介素 1β 的组织水平。通过 western 印迹分析进一步检查炎症反应揭示了 7 天的 BRB 处理降低了结肠组织内磷酸-IκBα 的水平。BRB 治疗也显着抑制结肠环加氧酶 2 水平，伴随着血浆前列腺素 E 2 的减少（276 比 34ng / ml）。这些发现表明 BRD 在 DSS 诱导的结肠损伤期间具有有效的抗炎作用。

PMID:21098643PM

DOI: 10.1093/carcin/bgq248

源自：

美国国家医学图书馆

美国国家卫生研究院

US National Library of Medicine/

Us National Institutes of Health/