学术报告 6
Cyanidin-3-rutinoside, a natural polyphenol antioxidant, selectively kills leukemic cells by induction of oxidative stress.
Cyanidin-3-rutinoside 是一种天然多酚抗氧化剂，通 过诱导氧化应激选择性杀伤白血病细胞。
原文链接官方网址：https://www.ncbi.nlm.nih.gov/pubmed
J Biol Chem. 2007 May 4;282(18):13468-76. Epub 2007 Mar 14.
J Biol Chem。2007 年 5 月 4 日; 282（18）：13468-76。电子书 2007 年 3 月 14 日。
Feng R1, Ni HM, Wang SY, Tourkova IL, Shurin MR, Harada H, Yin XM.
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh,
Pennsylvania 15261, USA.
匹兹堡大学医学院病理学系，宾夕法尼亚州匹兹堡 15261，美国。
Abstract
Anthocyanins are a group of naturally occurring phenolic compounds widely available in fruits and vegetables in human diets. They have broad biological activities including anti-mutagenesis and anticarcinogenesis, which are generally attributed to their antioxidant activities. We studied the effects and the
mechanisms of the most common type of anthocyanins, cyanidin-3-rutinoside, in several leukemia and lymphoma cell lines. We found that cyanidin-3-rutinoside extracted and purified from the black raspberry cultivar Jewel induced apoptosis in HL-60 cells in a dose- and time-dependent manner. Paradoxically, this compound induced the accumulation of peroxides, which are involved in the induction of apoptosis in HL-60 cells. In addition, cyanidin-3-rutinoside treatment resulted in reactive oxygen species (ROS)-dependent activation of p38 MAPK and JNK, which contributed to cell death by activating the mitochondrial pathway mediated by Bim. Down-regulation of Bim or overexpression of Bcl-2 or Bcl-x(L) considerably blocked apoptosis. Notably, cyanidin-3-rutinoside treatment did not lead to increased ROS accumulation in normal human peripheral blood mononuclear cells and had no cytotoxic effects on these cells. These results indicate that cyanidin-3-rutinoside has the potential to be used in leukemia therapy with the advantages of being widely available and selective against tumors.
花青素是一组天然存在的酚类化合物，广泛用于人类饮食中的水果和蔬菜。它们具有广泛的 生物学活性，包括抗诱变和抗癌作用，通常归因于它们的抗氧化活性。我们研究了几种白血 病和淋巴瘤细胞系中最常见类型花青素 cyanidin-3-rutinoside 的作用和机制。我们发现从黑 覆盆子栽培品种 Jewel 中提取并纯化的矢车菊素-3-芸香糖苷以剂量和时间依赖性方式诱导 HL-60 细胞凋亡。矛盾的是，这种化合物诱导过氧化物的积累，过氧化物参与诱导 HL-60 细 胞的凋亡。另外，花青素-3-芸香糖苷处理导致活性氧（ROS）依赖性激活 p38MAPK 和 JNK， 其通过激活由 Bim 介导的线粒体途径来促成细胞死亡。Bim 的下调或 Bcl-2 或 Bcl-x（L）的芸香糖苷有可能用于白血病治疗，具有广泛的可用性和对肿瘤的选择性。花青素-3-芸香糖苷处理不会导致正常人外周血单核细胞中 ROS 的积累增加，并且对这些细胞没有细胞毒性作用。 这些结果表明矢车菊色素-3-芸香糖苷有可能用于白血病治疗，具有广泛的可用性和对肿瘤的 选择性。花青素-3-芸香糖苷处理不会导致正常人外周血单核细胞中 ROS 的积累增加，并且 对这些细胞没有细胞毒性作用。这些结果表明矢车菊色素-3-芸香糖苷有可能用于白血病治疗， 具有广泛的可用性和对肿瘤的选择性。
PMID:17360708
源自：
美国国家医学图书馆
美国国家卫生研究院
US National Library of Medicine/
Us National Institutes of Health/
企业亮照