学术报告 7
A black raspberry extract inhibits proliferation and regulates apoptosis in cervical cancer cells.
黑覆盆子提取物可抑制宫颈癌细胞增殖并调节细胞凋亡。
原文链接官方网址：https://www.ncbi.nlm.nih.gov/pubmed
Gynecol Oncol. 2011 Nov;123(2):401-6. doi: 10.1016/j.ygyno.2011.07.023. Epub 2011
Aug 9
Gynecol Oncol。2011 年 11 月; 123（2）：401-6。doi：10.1016 / j.ygyno.2011.07.023。
Epub 2011 年 8 月 9 日。
Zhang Z1, Knobloch TJ, Seamon LG, Stoner GD, Cohn DE, Paskett ED, Fowler
College of Public Health, The Ohio State University, Columbus, OH, USA.
俄亥俄州立大学公共卫生学院，美国俄亥俄州哥伦布市。
Abstract
OBJECTIVE:
Cervical cancer is the second most common female cancer worldwide, and it remains a challenge to manage preinvasive and invasive lesions. Food-based cancer prevention entities, such as black raspberries and their derivatives, have
growth and tumor formation. Here, we extend the role of black raspberry-mediated
chemoprevention to that of cervical carcinogenesis.
目的： 宫颈癌是世界范围内第二大常见女性癌症，对于管理侵入性侵入性病变仍然是一项挑战。基于食 物的癌症预防实体，例如黑覆盆子及其衍生物，已经显示出显着抑制上皮癌细胞生长和肿瘤形成 的临床前模型的能力。在这里，我们将黑覆盆子介导的化学预防作用扩展到宫颈癌发生的作用。
METHODS:
Three human cervical cancer cell lines, HeLa (HPV16-/HPV18+, adenocarcinoma), SiHa (HPV16+/HPV18-, squamous cell carcinoma) and C-33A (HPV16-/HPV18-, squamous cell carcinoma), were treated with a lyophilized black raspberry ethanol extract (RO-ET) at 25, 50, 100 or 200μg/ml for 1, 3 and 5days, respectively. Cell proliferation was measured by WST1 (tetrazolium salt cleavage) assays. Flow cytometry (propidium iodide and Annexin V staining) and fluorescence microscopy analysis were used to measure apoptotic cell changes.
方法：
将三种人宫颈癌细胞系 HELA（HPV16- / HPV18 +，腺癌），SIHA（HPV16 + / HPV18-， 鳞状细胞癌）和 C-33A（HPV16- / HPV18-，鳞状细胞癌）用冷冻干燥的黑色覆盆子乙醇提取 物（RO-ET）分别在 25,50,100 或 200ΜG/ ML 下培养 1,3 和 5 天。通过 WST1（四唑盐切割）量凋亡细胞变化。
RESULTS:
We found that non-toxic levels of RO-ET significantly inhibited the growth of human cervical cancer cells, in a dose-dependent and time-dependent manner to a maximum of 54%, 52% and 67%, respectively (p<0.05). Furthermore, cell growth inhibition was persistent following short-term withdrawal of RO-ET from the culture medium. Flow cytometry and fluorescence microscopy demonstrated
RO-ET-induced apoptosis in all cell lines.
结果：
我们发现 RO-ET 的无毒水平以剂量依赖性和时间依赖性方式显着抑制人宫颈癌细胞的生长，最 大分别为 54％，52％和 67％（P<0.05） 。此外，细胞生长抑制在培养基中短期停用 RO-ET 后持续存在。流式细胞术和荧光显微镜显示 RO-ET 诱导的所有细胞系中的细胞凋亡。
CONCLUSION:
Black raspberries and their bioactive components represent promising candidates for future phytochemical-based mechanistic pathway-targeted cancer prevention strategies.
结论：
黑覆盆子及其生物活性成分代表未来基于植物化学机制途径的癌症预防策略的有希望的候选。
PMID:21831414
DOI:10.1016/j.ygyno.2011.07.023
源自：
美国国家医学图书馆
美国国家卫生研究院
US National Library of Medicine/
Us National Institutes of Health/
企业亮照